A simple method for clinical assay of superoxide dismutase.

A simple method for clinical assay of superoxide dismutase.

This assay for superoxide dismutase (SOD, EC 1.15.1.1) activity involves inhibition of nitroblue tetrazolium reduction, with xanthine-xanthine oxidase used as a superoxide generator. By using a reaction terminator, we can determine 40 samples within 55 min. One unit of activity of pure bovine liver Cu,ZnSOD and chicken liver MnSOD was expressed by 30 ng and 500 ng of protein, respectively. The ...

A modified spectrophotometric assay of superoxide dismutase.

A simple and rapid method for the assay of superoxide dismutase in biological samples is described. Present method takes advantage of the inhibition of NADH-dependent-nitroblue tetrazolium reduction by the dismutase. Inhibition of the chromogen formation by superoxide dismutase was linear with increase in enzyme concentrations. The chromogen extract in butanol was stable even up to 48 hr. Super...

The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase.

The rate of autoxidation of epinephrine and the sensitivity of this autoxidation to inhibition by superoxide dismutase were both augmented, as the pH was raised from 7.8 --f 10.2. 02-, generated by the xanthine oxidase reaction, caused the oxidation of epinephrine to adrenochrome and the yield of adrenochrome produced per 02introduced, increased with increasing pH in the range 7.8 + 10.2 and al...

The Role of Superoxide Anion in the Autoxidation of Epinephrine and a Simple Assay for Superoxide Dismutase*

The rate of autoxidation of epinephrine and the sensitivity of this autoxidation to inhibition by superoxide dismutase were both augmented, as the pH was raised from 7.8 --f 10.2. 02-, generated by the xanthine oxidase reaction, caused the oxidation of epinephrine to adrenochrome and the yield of adrenochrome produced per 02introduced, increased with increasing pH in the range 7.8 + 10.2 and al...

Superoxide dismutase Superoxide dismutase